Reporter

Part:BBa_K334002:Design

Designed by: Sean Kearney   Group: iGEM10_Purdue   (2010-10-25)


ODD with Luciferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 982


Design Notes

The part has to mimic the activity of HIF-1a in mammalian systems by degrading at normal oxygen concentrations and upregulating at high oxygen concentrations. The fusion of the degradation domain to a reporter theoretically enables this activity.


Source

It comes from the HIF1A gene in homo sapiens sapiens.

References